LOCUS       NC_000913               1254 bp    DNA     linear   BCT 13-FEB-2011
DEFINITION  Escherichia coli str. K-12 substr. MG1655 chromosome, complete
            genome.
ACCESSION   NC_000913 REGION: complement(361150..362403)
VERSION     NC_000913.2  GI:49175990
DBLINK      Project: 57779
KEYWORDS    .
SOURCE      Escherichia coli str. K-12 substr. MG1655
  ORGANISM  Escherichia coli str. K-12 substr. MG1655
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Bury-Mone,S., Nomane,Y., Reymond,N., Barbet,R., Jacquet,E.,
            Imbeaud,S., Jacq,A. and Bouloc,P.
  TITLE     Global analysis of extracytoplasmic stress signaling in Escherichia
            coli
  JOURNAL   PLoS Genet. 5 (9), E1000651 (2009)
   PUBMED   19763168
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Oberto,J., Nabti,S., Jooste,V., Mignot,H. and Rouviere-Yaniv,J.
  TITLE     The HU regulon is composed of genes responding to anaerobiosis,
            acid stress, high osmolarity and SOS induction
  JOURNAL   PLoS ONE 4 (2), E4367 (2009)
   PUBMED   19194530
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1254)
  AUTHORS   Inoue,T., Shingaki,R., Hirose,S., Waki,K., Mori,H. and Fukui,K.
  TITLE     Genome-wide screening of genes required for swarming motility in
            Escherichia coli K-12
  JOURNAL   J. Bacteriol. 189 (3), 950-957 (2007)
   PUBMED   17122336
REFERENCE   4  (bases 1 to 1254)
  AUTHORS   Lu,P., Vogel,C., Wang,R., Yao,X. and Marcotte,E.M.
  TITLE     Absolute protein expression profiling estimates the relative
            contributions of transcriptional and translational regulation
  JOURNAL   Nat. Biotechnol. 25 (1), 117-124 (2007)
   PUBMED   17187058
REFERENCE   5  (bases 1 to 1254)
  AUTHORS   Joyce,A.R., Reed,J.L., White,A., Edwards,R., Osterman,A., Baba,T.,
            Mori,H., Lesely,S.A., Palsson,B.O. and Agarwalla,S.
  TITLE     Experimental and computational assessment of conditionally
            essential genes in Escherichia coli
  JOURNAL   J. Bacteriol. 188 (23), 8259-8271 (2006)
   PUBMED   17012394
REFERENCE   6  (bases 1 to 1254)
  AUTHORS   Lasserre,J.P., Beyne,E., Pyndiah,S., Lapaillerie,D., Claverol,S.
            and Bonneu,M.
  TITLE     A complexomic study of Escherichia coli using two-dimensional blue
            native/SDS polyacrylamide gel electrophoresis
  JOURNAL   Electrophoresis 27 (16), 3306-3321 (2006)
   PUBMED   16858726
REFERENCE   7  (bases 1 to 1254)
  AUTHORS   Han,M.J. and Lee,S.Y.
  TITLE     The Escherichia coli proteome: past, present, and future prospects
  JOURNAL   Microbiol. Mol. Biol. Rev. 70 (2), 362-439 (2006)
   PUBMED   16760308
REFERENCE   8  (bases 1 to 1254)
  AUTHORS   Arifuzzaman,M., Maeda,M., Itoh,A., Nishikata,K., Takita,C.,
            Saito,R., Ara,T., Nakahigashi,K., Huang,H.C., Hirai,A., Tsuzuki,K.,
            Nakamura,S., Altaf-Ul-Amin,M., Oshima,T., Baba,T., Yamamoto,N.,
            Kawamura,T., Ioka-Nakamichi,T., Kitagawa,M., Tomita,M., Kanaya,S.,
            Wada,C. and Mori,H.
  TITLE     Large-scale identification of protein-protein interaction of
            Escherichia coli K-12
  JOURNAL   Genome Res. 16 (5), 686-691 (2006)
   PUBMED   16606699
REFERENCE   9  (bases 1 to 1254)
  AUTHORS   Riley,M., Abe,T., Arnaud,M.B., Berlyn,M.K., Blattner,F.R.,
            Chaudhuri,R.R., Glasner,J.D., Horiuchi,T., Keseler,I.M., Kosuge,T.,
            Mori,H., Perna,N.T., Plunkett,G. III, Rudd,K.E., Serres,M.H.,
            Thomas,G.H., Thomson,N.R., Wishart,D. and Wanner,B.L.
  TITLE     Escherichia coli K-12: a cooperatively developed annotation
            snapshot--2005
  JOURNAL   Nucleic Acids Res. 34 (1), 1-9 (2006)
   PUBMED   16397293
  REMARK    Publication Status: Online-Only
REFERENCE   10 (bases 1 to 1254)
  AUTHORS   Saier,M.H. Jr., Tran,C.V. and Barabote,R.D.
  TITLE     TCDB: the Transporter Classification Database for membrane
            transport protein analyses and information
  JOURNAL   Nucleic Acids Res. 34 (DATABASE ISSUE), D181-D186 (2006)
   PUBMED   16381841
REFERENCE   11 (bases 1 to 1254)
  AUTHORS   Herzberg,M., Kaye,I.K., Peti,W. and Wood,T.K.
  TITLE     YdgG (TqsA) controls biofilm formation in Escherichia coli K-12
            through autoinducer 2 transport
  JOURNAL   J. Bacteriol. 188 (2), 587-598 (2006)
   PUBMED   16385049
REFERENCE   12 (bases 1 to 1254)
  AUTHORS   Baba,T., Ara,T., Hasegawa,M., Takai,Y., Okumura,Y., Baba,M.,
            Datsenko,K.A., Tomita,M., Wanner,B.L. and Mori,H.
  TITLE     Construction of Escherichia coli K-12 in-frame, single-gene
            knockout mutants: the Keio collection
  JOURNAL   Mol. Syst. Biol. 2, 2006 (2006)
   PUBMED   16738554
  REMARK    Publication Status: Online-Only
REFERENCE   13 (bases 1 to 1254)
  AUTHORS   Granseth,E., Daley,D.O., Rapp,M., Melen,K. and von Heijne,G.
  TITLE     Experimentally constrained topology models for 51,208 bacterial
            inner membrane proteins
  JOURNAL   J. Mol. Biol. 352 (3), 489-494 (2005)
   PUBMED   16120447
REFERENCE   14 (bases 1 to 1254)
  AUTHORS   Daley,D.O., Rapp,M., Granseth,E., Melen,K., Drew,D. and von
            Heijne,G.
  TITLE     Global topology analysis of the Escherichia coli inner membrane
            proteome
  JOURNAL   Science 308 (5726), 1321-1323 (2005)
   PUBMED   15919996
REFERENCE   15 (bases 1 to 1254)
  AUTHORS   Weber,H., Polen,T., Heuveling,J., Wendisch,V.F. and Hengge,R.
  TITLE     Genome-wide analysis of the general stress response network in
            Escherichia coli: sigmaS-dependent genes, promoters, and sigma
            factor selectivity
  JOURNAL   J. Bacteriol. 187 (5), 1591-1603 (2005)
   PUBMED   15716429
REFERENCE   16 (bases 1 to 1254)
  AUTHORS   Butland,G., Peregrin-Alvarez,J.M., Li,J., Yang,W., Yang,X.,
            Canadien,V., Starostine,A., Richards,D., Beattie,B., Krogan,N.,
            Davey,M., Parkinson,J., Greenblatt,J. and Emili,A.
  TITLE     Interaction network containing conserved and essential protein
            complexes in Escherichia coli
  JOURNAL   Nature 433 (7025), 531-537 (2005)
   PUBMED   15690043
REFERENCE   17 (bases 1 to 1254)
  AUTHORS   Marino-Ramirez,L., Minor,J.L., Reading,N. and Hu,J.C.
  TITLE     Identification and mapping of self-assembling protein domains
            encoded by the Escherichia coli K-12 genome by use of lambda
            repressor fusions
  JOURNAL   J. Bacteriol. 186 (5), 1311-1319 (2004)
   PUBMED   14973045
REFERENCE   18 (bases 1 to 1254)
  AUTHORS   Sabina,J., Dover,N., Templeton,L.J., Smulski,D.R., Soll,D. and
            LaRossa,R.A.
  TITLE     Interfering with different steps of protein synthesis explored by
            transcriptional profiling of Escherichia coli K-12
  JOURNAL   J. Bacteriol. 185 (20), 6158-6170 (2003)
   PUBMED   14526028
REFERENCE   19 (bases 1 to 1254)
  AUTHORS   Polen,T., Rittmann,D., Wendisch,V.F. and Sahm,H.
  TITLE     DNA microarray analyses of the long-term adaptive response of
            Escherichia coli to acetate and propionate
  JOURNAL   Appl. Environ. Microbiol. 69 (3), 1759-1774 (2003)
   PUBMED   12620868
REFERENCE   20 (bases 1 to 1254)
  AUTHORS   Pomposiello,P.J., Bennik,M.H. and Demple,B.
  TITLE     Genome-wide transcriptional profiling of the Escherichia coli
            responses to superoxide stress and sodium salicylate
  JOURNAL   J. Bacteriol. 183 (13), 3890-3902 (2001)
   PUBMED   11395452
REFERENCE   21 (bases 1 to 1254)
  AUTHORS   Smulski,D.R., Huang,L.L., McCluskey,M.P., Reeve,M.J., Vollmer,A.C.,
            Van Dyk,T.K. and LaRossa,R.A.
  TITLE     Combined, functional genomic-biochemical approach to intermediary
            metabolism: interaction of acivicin, a glutamine amidotransferase
            inhibitor, with Escherichia coli K-12
  JOURNAL   J. Bacteriol. 183 (11), 3353-3364 (2001)
   PUBMED   11344143
REFERENCE   22 (bases 1 to 1254)
  AUTHORS   Serres,M.H., Gopal,S., Nahum,L.A., Liang,P., Gaasterland,T. and
            Riley,M.
  TITLE     A functional update of the Escherichia coli K-12 genome
  JOURNAL   Genome Biol. 2 (9), RESEARCH0035 (2001)
   PUBMED   11574054
REFERENCE   23 (bases 1 to 1254)
  AUTHORS   Blattner,F.R., Plunkett,G. III, Bloch,C.A., Perna,N.T., Burland,V.,
            Riley,M., Collado-Vides,J., Glasner,J.D., Rode,C.K., Mayhew,G.F.,
            Gregor,J., Davis,N.W., Kirkpatrick,H.A., Goeden,M.A., Rose,D.J.,
            Mau,B. and Shao,Y.
  TITLE     The complete genome sequence of Escherichia coli K-12
  JOURNAL   Science 277 (5331), 1453-1474 (1997)
   PUBMED   9278503
REFERENCE   24 (bases 1 to 1254)
  AUTHORS   Burland,V., Plunkett,G. III, Sofia,H.J., Daniels,D.L. and
            Blattner,F.R.
  TITLE     Analysis of the Escherichia coli genome VI: DNA sequence of the
            region from 92.8 through 100 minutes
  JOURNAL   Nucleic Acids Res. 23 (12), 2105-2119 (1995)
   PUBMED   7610040
REFERENCE   25 (bases 1 to 1254)
  AUTHORS   Sofia,H.J., Burland,V., Daniels,D.L., Plunkett,G. III and
            Blattner,F.R.
  TITLE     Analysis of the Escherichia coli genome. V. DNA sequence of the
            region from 76.0 to 81.5 minutes
  JOURNAL   Nucleic Acids Res. 22 (13), 2576-2586 (1994)
   PUBMED   8041620
REFERENCE   26 (bases 1 to 1254)
  AUTHORS   Blattner,F.R., Burland,V., Plunkett,G. III, Sofia,H.J. and
            Daniels,D.L.
  TITLE     Analysis of the Escherichia coli genome. IV. DNA sequence of the
            region from 89.2 to 92.8 minutes
  JOURNAL   Nucleic Acids Res. 21 (23), 5408-5417 (1993)
   PUBMED   8265357
REFERENCE   27 (bases 1 to 1254)
  AUTHORS   Burland,V., Plunkett,G. III, Daniels,D.L. and Blattner,F.R.
  TITLE     DNA sequence and analysis of 136 kilobases of the Escherichia coli
            genome: organizational symmetry around the origin of replication
  JOURNAL   Genomics 16 (3), 551-561 (1993)
   PUBMED   7686882
REFERENCE   28 (bases 1 to 1254)
  AUTHORS   Arnaud,M., Berlyn,M.K.B., Blattner,F.R., Galperin,M.Y.,
            Glasner,J.D., Horiuchi,T., Kosuge,T., Mori,H., Perna,N.T.,
            Plunkett,G. III, Riley,M., Rudd,K.E., Serres,M.H., Thomas,G.H. and
            Wanner,B.L.
  TITLE     Workshop on Annotation of Escherichia coli K-12
  JOURNAL   Unpublished
  REMARK    Woods Hole, Mass., on 14-18 November 2003 (sequence corrections)
REFERENCE   29 (bases 1 to 1254)
  AUTHORS   Glasner,J.D., Perna,N.T., Plunkett,G. III, Anderson,B.D.,
            Bockhorst,J., Hu,J.C., Riley,M., Rudd,K.E. and Serres,M.H.
  TITLE     ASAP: Escherichia coli K-12 strain MG1655 version m56
  JOURNAL   Unpublished
  REMARK    ASAP download 10 June 2004 (annotation updates)
REFERENCE   30 (bases 1 to 1254)
  AUTHORS   Hayashi,K., Morooka,N., Mori,H. and Horiuchi,T.
  TITLE     A more accurate sequence comparison between genomes of Escherichia
            coli K12 W3110 and MG1655 strains
  JOURNAL   Unpublished
  REMARK    GenBank accessions AG613214 to AG613378 (sequence corrections)
REFERENCE   31 (bases 1 to 1254)
  AUTHORS   Perna,N.T.
  TITLE     Escherichia coli K-12 MG1655 yqiK-rfaE intergenic region, genomic
            sequence correction
  JOURNAL   Unpublished
  REMARK    GenBank accession AY605712 (sequence corrections)
REFERENCE   32 (bases 1 to 1254)
  AUTHORS   Rudd,K.E.
  TITLE     A manual approach to accurate translation start site annotation: an
            E. coli K-12 case study
  JOURNAL   Unpublished
REFERENCE   33 (bases 1 to 1254)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2010) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   34 (bases 1 to 1254)
  AUTHORS   Rudd,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2010) Department of Biochemistry and Molecular
            Biology, University of Miami Miller School of Medicine, 118 Gautier
            Bldg., Miami, FL 33136, USA
  REMARK    Annotation update from ecogene.org as a multi-database
            collaboration
REFERENCE   35 (bases 1 to 1254)
  AUTHORS   Rudd,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2007) Department of Biochemistry and Molecular
            Biology, University of Miami Miller School of Medicine, 118 Gautier
            Bldg., Miami, FL 33136, USA
  REMARK    Annotation update from ecogene.org as a multi-database
            collaboration
REFERENCE   36 (bases 1 to 1254)
  AUTHORS   Plunkett,G. III.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2006) Laboratory of Genetics, University of
            Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
  REMARK    Protein updates by submitter
REFERENCE   37 (bases 1 to 1254)
  AUTHORS   Plunkett,G. III.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2004) Laboratory of Genetics, University of
            Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
  REMARK    Sequence update by submitter
REFERENCE   38 (bases 1 to 1254)
  AUTHORS   Plunkett,G. III.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-1998) Laboratory of Genetics, University of
            Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
REFERENCE   39 (bases 1 to 1254)
  AUTHORS   Blattner,F.R. and Plunkett,G. III.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-1997) Laboratory of Genetics, University of
            Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
REFERENCE   40 (bases 1 to 1254)
  AUTHORS   Blattner,F.R. and Plunkett,G. III.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-1997) Laboratory of Genetics, University of
            Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence is identical to U00096.
            On Jun 24, 2004 this sequence version replaced gi:16127994.
            Current U00096 annotation updates are derived from EcoGene
            http://ecogene.org. Suggestions for updates can be sent to Dr.
            Kenneth Rudd (krudd@miami.edu). These updates are being generated
            from a collaboration that also includes ASAP/ERIC, the Coli Genetic
            Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Escherichia coli str. K-12 substr. MG1655"
                     /mol_type="genomic DNA"
                     /strain="K-12"
                     /sub_strain="MG1655"
                     /db_xref="taxon:511145"
     gene            1..1254
                     /gene="lacY"
                     /locus_tag="b0343"
                     /gene_synonym="ECK0340; JW0334"
                     /db_xref="ECOCYC:EG10526"
                     /db_xref="EcoGene:EG10526"
                     /db_xref="GeneID:949083"
     CDS             1..1254
                     /gene="lacY"
                     /locus_tag="b0343"
                     /gene_synonym="ECK0340; JW0334"
                     /function="transport; Transport of small molecules:
                     Carbohydrates, organic acids, alcohols"
                     /function="1.1.1 metabolism; carbon utilization; carbon
                     compounds"
                     /function="4.2.A.1 transport; Electrochemical potential
                     driven transporters; Porters (Uni-, Sym- and Antiporters);
                     The Major Facilitator Superfamily (MFS)"
                     /function="6.1 cell structure; membrane"
                     /function="7.3 location of gene products; inner membrane"
                     /function="4.S.99 transport; substrate; lactose"
                     /GO_component="GO:0019866 - organelle inner membrane;
                     GO:0009274 - peptidoglycan-based cell wall"
                     /GO_process="GO:0016052 - carbohydrate catabolic process"
                     /note="galactoside permease (lactose permease, M protein)
                     (MFS family)"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="NP_414877.1"
                     /db_xref="GI:16128328"
                     /db_xref="ASAP:ABE-0001181"
                     /db_xref="UniProtKB/Swiss-Prot:P02920"
                     /db_xref="ECOCYC:EG10526"
                     /db_xref="EcoGene:EG10526"
                     /db_xref="GeneID:949083"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
ORIGIN      
        1 atgtactatt taaaaaacac aaacttttgg atgttcggtt tattcttttt cttttacttt
       61 tttatcatgg gagcctactt cccgtttttc ccgatttggc tacatgacat caaccatatc
      121 agcaaaagtg atacgggtat tatttttgcc gctatttctc tgttctcgct attattccaa
      181 ccgctgtttg gtctgctttc tgacaaactc gggctgcgca aatacctgct gtggattatt
      241 accggcatgt tagtgatgtt tgcgccgttc tttattttta tcttcgggcc actgttacaa
      301 tacaacattt tagtaggatc gattgttggt ggtatttatc taggcttttg ttttaacgcc
      361 ggtgcgccag cagtagaggc atttattgag aaagtcagcc gtcgcagtaa tttcgaattt
      421 ggtcgcgcgc ggatgtttgg ctgtgttggc tgggcgctgt gtgcctcgat tgtcggcatc
      481 atgttcacca tcaataatca gtttgttttc tggctgggct ctggctgtgc actcatcctc
      541 gccgttttac tctttttcgc caaaacggat gcgccctctt ctgccacggt tgccaatgcg
      601 gtaggtgcca accattcggc atttagcctt aagctggcac tggaactgtt cagacagcca
      661 aaactgtggt ttttgtcact gtatgttatt ggcgtttcct gcacctacga tgtttttgac
      721 caacagtttg ctaatttctt tacttcgttc tttgctaccg gtgaacaggg tacgcgggta
      781 tttggctacg taacgacaat gggcgaatta cttaacgcct cgattatgtt ctttgcgcca
      841 ctgatcatta atcgcatcgg tgggaaaaac gccctgctgc tggctggcac tattatgtct
      901 gtacgtatta ttggctcatc gttcgccacc tcagcgctgg aagtggttat tctgaaaacg
      961 ctgcatatgt ttgaagtacc gttcctgctg gtgggctgct ttaaatatat taccagccag
     1021 tttgaagtgc gtttttcagc gacgatttat ctggtctgtt tctgcttctt taagcaactg
     1081 gcgatgattt ttatgtctgt actggcgggc aatatgtatg aaagcatcgg tttccagggc
     1141 gcttatctgg tgctgggtct ggtggcgctg ggcttcacct taatttccgt gttcacgctt
     1201 agcggccccg gcccgctttc cctgctgcgt cgtcaggtga atgaagtcgc ttaa
//
